Fig. 2

Effect of various biomaterial wound care products on healing in vivo. A (left) Schematic showing four 6 mm diameter full thickness wounds on the backs of db/db mice that were treated with HKM, another biomaterial-based wound care product, or no treatment (control) in randomised locations. Image created with Biorender.com. Representative images of the four wounds at week 0 before application of treatment (middle-left), wounds treated and topped with secondary dressings (middle-right), and wounds after several weekly treatments (right), in this case HKM (i), control (ii), and bovine dermis (iii) at week 3 post-operation. B Bar graph showing average time to complete closure for each treatment applied. *p < 0.05, ****p < 0.0001 by one-way ANOVA with Tukey's multiple comparisons. C Healing trajectories of wounds on mice treated with control (black circle, n = 12), amniotic membrane (blue square, n = 12), or HKM (gold triangle, n = 12). D Healing trajectories of wounds on mice treated with control (black circle, n = 12), bovine dermal collagen (blue square, n = 12), or HKM (gold triangle, n = 12). E Healing trajectories of wounds on mice treated with control (black circle, n = 16), porcine small intestinal submucosa (blue square, n = 16), or HKM (gold triangle, n = 16). Symbols indicate statistical significance of HKM compared to other treatments: *p < 0.05 vs. control, **p < 0.01 vs. control, ****p < 0.0001 vs. control, ^#p < 0.05 vs. corresponding comparative advanced wound care product, ^##p < 0.01 vs. corresponding comparative advanced wound care product, ^####p < 0.0001 vs. corresponding comparative advanced wound care product by two-way analysis of variance (ANOVA), paired by mouse, with Tukey's multiple comparisons at each timepoint [93]. Creative Commons Attribution License